How do protein–RNA interactions influence the structure and function of virus capsids?

Sourav PanOctober 28, 2024

Answered

Protein–RNA interactions play a crucial role in the structure and function of virus capsids in several ways:

1. Capsid Assembly: The interaction between viral proteins and RNA is fundamental to the assembly of the capsid. Many viruses utilize their RNA genome as a scaffold around which the protein subunits assemble. The specific binding sites on the RNA help guide the correct arrangement of proteins, ensuring that the capsid forms a stable and functional structure.
2. Stability of the Capsid: Protein–RNA interactions contribute to the overall stability of the viral capsid. The binding of RNA can enhance the structural integrity of the capsid by providing additional contacts between protein subunits. This stabilization is particularly important for protecting the viral genome from degradation in the extracellular environment.
3. Conformational Changes: The binding of RNA can induce conformational changes in the capsid proteins, which may be necessary for the proper assembly of the capsid or for the activation of viral functions. These changes can affect how the capsid interacts with host cell receptors during the infection process.
4. Genome Packaging: The specificity of protein–RNA interactions is critical for the selective packaging of the viral genome into the capsid. The proteins recognize specific sequences or structures within the RNA, ensuring that only the correct viral RNA is encapsulated, which is essential for the production of infectious viral particles.
5. Release of the Genome: During the infection process, the interaction between the capsid proteins and RNA can facilitate the release of the viral genome into the host cell. This may involve conformational changes in the capsid that expose the RNA or allow it to be released in a manner that is conducive to replication and translation.
6. Regulation of Viral Functions: Protein–RNA interactions can also play a role in regulating various viral functions, such as replication and translation. The binding of RNA to specific protein domains can influence the activity of viral enzymes or other proteins involved in the viral life cycle.
7. Antigenic Variation: The interactions between capsid proteins and RNA can influence the antigenic properties of the virus. Variations in the RNA sequence or structure can lead to changes in the protein conformation, potentially affecting how the virus is recognized by the host immune system.