How can a liquid be drawn into a pipette safely?
How can a liquid be drawn into a pipette safely?
Answered
To draw liquid into a pipette safely, ensure you use the correct pipette and follow these steps: 1. Attach a clean, suitable pipette tip (if using a disposable pipette). 2. Avoid touching the inside of the tip to prevent contamination. 3. If using a manual pipette, squeeze the bulb or depress the plunger (depending on the type) to create suction. Immerse the tip of the pipette into the liquid at a slight angle to avoid drawing air bubbles. 4. Slowly release the suction mechanism to draw the liquid into the pipette, ensuring the liquid reaches the appropriate level, aligned with the meniscus. Never aspirate liquid too quickly, as it can cause air bubbles or inaccurate measurements. 5. Always avoid drawing liquid above the graduation mark, and ensure you use appropriate personal protective equipment (PPE) to handle chemicals or biological materials.
BNO Team