1. Write and account on enumeration of bacteria in a food sample.

The enumeration of bacteria in a food sample is a multistep process that includes:

• Aseptic collection and homogenization of the food sample.
• Serial dilution to achieve a countable range of bacterial colonies.
• Plating using methods such as spread plate, pour plate, membrane filtration, or MPN.
• Incubation under conditions optimized for the target organisms.
• Counting colonies and calculating the bacterial concentration as CFU per unit weight or volume.