16 Difference Between Brightfield and Fluorescence Microscope.

Published on May 20, 2024 Written by BNO Team

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In My previous notes i have already discussed about Brightfield Microscope and Fluorescence Microscope, their working principle, parts, definition, application, advantages, disadvantages and light path. You can check them out.

Difference Between Brightfield Microscope and Fluorescence Microscope.

Topic	Brightfield Microscope	Fluorescence Microscope
Invented by	Antonie van Leeuwenhoek (1632–1724)	Antonie van Leeuwenhoek (1632–1724)
Fluorochromes	Not required	Required.
Specimen Image	Form dark image against bright background.	Form bright image against dark background.
Light Source	Light source may be natural or artificial.	Light source must be artificial.
Exciter Filter	Not required.	Required.
Dichroic Filter	Not Required.	Required.
Emission filter	Absent	Present
Staining	The specimen staining is done with the safranin, crystal violet, etc.	The specimen staining is done with fluorochromes or Fluorescent dey.
Specimen Preparation	Quick and simple processes.	Long and complex processes.
Expensive	It is cheaper.	Expensive and Specimen preparation is a costly process.
Image Detector	Absent	Present
Image Visualization	Image is visualize through the eyepiece.	Image is visualize on a digital display.
Protein Localization	It can not localize specific proteins within the cell.	It can localize specific proteins within the cell.
multicolor staining	Not allowed	Allowed
Living cell study	Not possible	Possible
fluorescence and phosphorescence	Absent	Present

Brightfield Microscope Image

Fluorescence Microscope Image

Reference

• https://microbiologynote.com/fluorescence-microscope-principle-definition-uses-parts/
• https://microbiologynote.com/bright-field-microscope-definition-parts-working-principle-application/
• https://www.news-medical.net/life-sciences/Fluorescence-Microscopy-vs-Light-Microscopy.aspx

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