GATE Biotechnology Mock Test 3

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GATE Biotechnology Mock Test 3

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Program used for essentially local similarity search is
Q32 Match Group I with Group II: Group I: P. Fibronectin, Q. Insulin, R. α-Macroglobulin, S. Transferrin. Group II: 1. Uptake of amino acids and glucose; 2. Trypsin inhibitor; 3. Binds iron; 4. Cell attachment to substratum.
Q33 Match promoters in Group I with tissues in Group II: Group I: P. α-Amylase, Q. Glutenin, R. Phaseolin, S. Patatin. Group II: 1. Endosperm; 2. Tuber; 3. Aleurone; 4. Cotyledon.
Q34 Assertion (A): T₄ DNA ligase can catalyze blunt-end ligation more efficiently than E. coli DNA ligase. Reason (R): The ligation efficiency of T₄ DNA ligase can be increased with PEG and ficoll.
Q35 The turnover numbers (k₋ₐₜ) for enzymes E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means
Q36 Match items in Group I (Vectors) with Group II (Maximum DNA packaging): P. λ phage; Q. BACs; R. PACs; S. cosmid. Group II: 1. 35–45 kb; 2. 100–300 kb; 3. ≤300 kb; 4. 5–25 kb.
Q37 Match Group I with Group II: P. Staphylococcus aureus, Q. Candida albicans, R. Mycobacterium tuberculosis, S. Lactobacillus lactis. Group II: 1. Staphylocins; 2. Bacteriocins; 3. Methicillin resistance; 4. Isoniazid.
Q38 A mutant Gα protein with increased GTPase activity would
Q39 Dizygotic twins are connected to a single placenta during their embryonic development. These twins
Q40 The dissociation constant Kₙ for ligand binding to the receptor is 10⁻⁷ M. The concentration of ligand required for occupying 10% of receptors is
Q41 Receptor R is overexpressed in CHO cells (6×10⁷ cells). Incubated with radioligand (100 cpm/pmol), pellet counts = 1000 cpm. Assuming one ligand binds one receptor, average receptors per cell is
Q42 A cell has five molecules of a rare mRNA amid 4×10⁵ total mRNAs. How many clones must be screened for 99% probability of finding at least one recombinant cDNA of the rare mRNA?

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