- A) (A) P-1, Q-4, R-2, S-3
- B) (B) P-2, Q-3, R-1, S-4
- C) (C) P-3, Q-1, R-4, S-2
- D) (D) P-1, Q-2, R-4, S-3
GATE Biotechnology
Total MCQ: 47
12 Q35 The turnover numbers (k₋ₐₜ) for enzymes E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means
- A) (A) E1 binds substrate with higher affinity
- B) (B) velocities could be equal if [E2] = 10×[E1]
- C) (C) velocity of E1 > velocity of E2
- D) (D) velocity of E1 < velocity of E2
13 Q36 Match items in Group I (Vectors) with Group II (Maximum DNA packaging): P. λ phage; Q. BACs; R. PACs; S. cosmid. Group II: 1. 35–45 kb; 2. 100–300 kb; 3. ≤300 kb; 4. 5–25 kb.
- A) (A) P-3, Q-4, R-1, S-2
- B) (B) P-1, Q-3, R-2, S-4
- C) (C) P-4, Q-3, R-2, S-1
- D) (D) P-1, Q-2, R-3, S-4
14 Q34 Assertion (A): T₄ DNA ligase can catalyze blunt-end ligation more efficiently than E. coli DNA ligase. Reason (R): The ligation efficiency of T₄ DNA ligase can be increased with PEG and ficoll.
- A) (A) both (A) and (R) true; (R) is correct
- B) (B) both true; (R) not the correct reason
- C) (C) (A) true, (R) false
- D) (D) (A) false, (R) true
15 Q32 Match Group I with Group II: Group I: P. Fibronectin, Q. Insulin, R. α-Macroglobulin, S. Transferrin. Group II: 1. Uptake of amino acids and glucose; 2. Trypsin inhibitor; 3. Binds iron; 4. Cell attachment to substratum.
- A) (A) P-2, Q-1, R-4, S-3
- B) (B) P-3, Q-2, R-1, S-4
- C) (C) P-4, Q-2, R-1, S-3
- D) (D) P-4, Q-1, R-2, S-3
16 Q33 Match promoters in Group I with tissues in Group II: Group I: P. α-Amylase, Q. Glutenin, R. Phaseolin, S. Patatin. Group II: 1. Endosperm; 2. Tuber; 3. Aleurone; 4. Cotyledon.
- A) (A) P-3, Q-1, R-4, S-2
- B) (B) P-3, Q-4, R-1, S-2
- C) (C) P-4, Q-2, R-1, S-3
- D) (D) P-1, Q-3, R-2, S-4
17 Match the chemicals in Group I with their possible type/class in Group II. Group I: P. Picolinam, Q. Zeatin, R. Thiamine, S. Glutamine. Group II: 1. Vitamin; 2. Auxin; 3. Amino acid; 4. Cytokinin.
- A) (A) P-2, Q-4, R-1, S-3
- B) (B) P-4, Q-1, R-2, S-3
- C) (C) P-3, Q-1, R-2, S-4
- D) (D) P-4, Q-2, R-1, S-3
18 Determine the correctness of the following Assertion (A) and Reason (R): Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on colour development by converting tetrazolium soluble salt to insoluble formazan.
- A) both (A) and (R) true, (R) is correct reason for (A)
- B) both (A) and (R) true, (R) not correct reason for (A)
- C) (A) true, (R) false
- D) (A) false, (R) true
19 Match the following antibiotics in Group I with their mode of action in Group II. Group I: P. Chloramphenicol, Q. Norfloxacin, R. Puromycin, S. Rifampicin. Group II: 1. Binds to DNA gyrase; 2. Binds to RNA polymerase; 3. Inhibits peptidyl transferase; 4. Mimics aminoacyl-tRNA.
- A) (A) P-1, Q-3, R-2, S-4
- B) (B) P-3, Q-1, R-2, S-4
- C) (C) P-3, Q-1, R-4, S-2
- D) (D) P-4, Q-2, R-3, S-1
20 A roller-bottle culture vessel, cylindrical (r = 10 cm, l = 20 cm), is fitted with a spiral film (L = 30 cm, w = 20 cm). If the film can support 10⁸ anchorage-dependent cells per cm², the increase in surface area and additional number of cells that can grow are
- A) 1200 cm² and 1.2 × 10⁷ cells
- B) 600 cm² and 6 × 10⁸ cells
- C) 600 cm² and 8 300 cells
- D) 1200 cm² and 8 300 cells