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what is the name of the kinetic intermediate in protein folding where secondary structure elements have formed their regular H-bonding patterns and there has been collapse to separate most nonpolar side chains into the protein interior and most hydrophilic side chains to the protein exterior, but the side chain packing has not yet been optimized to achieve a tightly packed protein interior with a crystalline density?

what is the name of the kinetic intermediate in protein folding where secondary structure elements have formed their regular H-bonding patterns and there has been collapse to separate most nonpolar side chains into the protein interior and most hydrophilic side chains to the protein exterior, but the side chain packing has not yet been optimized … Read more

1. If you did “overload” your Ni+2 Agarose column with a GCE sample that contained too much rGFP, would you expect to see a band in the W2 lane of the Western Blot? If so what is it’s MW? Would you expect to see a band in the E2 lane? If so what is it’s MW? 2. Chromatography columns have a limited protein binding capacity. During the Ni2+ agarose column lab, you shouldnt have overloaded the column. If you did the W2 fraction may have fluoresced slightly. If you see a band in the Western Blot W2 lane, what does this data say about the physical protein structure of rGFP in that lane? Give a possible MW for this band based upon the data.

1. If you did “overload” your Ni+2 Agarose column with a GCE sample that contained too much rGFP, would you expect to see a band in the W2 lane of the Western Blot? If so what is it’s MW? Would you expect to see a band in the E2 lane? If so what is it’s … Read more

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