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1 What drives the directionality of reactions in glycolysis?

A: Temperature and pressure

B: Concentration of reactants and products

C: Enzyme concentration

D: All of these factors

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2 Which compound serves as an allosteric regulator derived from glycolytic intermediates?

A: Lactate

B: Acetyl-CoA

C: 2,3-Bisphosphoglycerate

D: Fructose-1,6-bisphosphate

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3 What type of reaction occurs during substrate-level phosphorylation in glycolysis?

A: Oxidation-reduction reaction

B: Hydrolysis reaction

C: Phosphorylation reaction

D: Condensation reaction

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4 What is the role of NAD+ in glycolysis?

A: It acts as an electron donor.

B: It gets reduced to NADH.

C: It generates ATP directly.

D: It phosphorylates glucose.

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5 Which glycolytic intermediate can be converted into glycerol for lipid synthesis?

A: Fructose-6-phosphate

B: Glyceraldehyde-3-phosphate

C: Dihydroxyacetone phosphate

D: Pyruvate

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6 Which of the following enzymes catalyzes a key regulatory step in glycolysis?

A: Hexokinase

B: Phosphofructokinase-1

C: Pyruvate kinase

D: All of the above

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7 What happens to pyruvate when oxygen is present?

A: It is converted to lactate.

B: It enters the TCA cycle.

C: It is converted to ethanol.

D: It remains in the cytoplasm.

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8 What is the overall reaction of glycolysis?

A: Glucose + 2 NAD+ + 2 ADP + 2 Pi → 2 Pyruvate + 2 NADH + 2 H+ + 2 ATP + 2 H2O

B: Glucose + O2 → CO2 + H2O + ATP

C: Pyruvate + NADH → Lactate + NAD+

D: Glucose + ATP → Glucose-6-phosphate + ADP

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9 Which of the following sugars can enter glycolysis?

A: Sucrose

B: Fructose

C: Galactose

D: All of the above

Biochemistry MCQ
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10 What does the term “ATP payoff phase” refer to in glycolysis?

A: The phase where ATP is consumed

B: The phase where ATP is generated

C: The phase where glucose is broken down

D: The phase where NADH is produced

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