Which of the following technological advances have made high-throughput sequencing possible?

Sourav PanSeptember 18, 2024

Answered

Several technological advances have made high-throughput sequencing possible, including: 1) Improved sequencing chemistry: Innovations such as reversible terminator chemistry have enabled the sequencing of millions of DNA fragments simultaneously. 2) Automation: Advances in robotic systems and automation have streamlined sample preparation, sequencing, and data processing, reducing manual labor and increasing throughput. 3) High-performance computing: Enhanced computational power and algorithms are crucial for analyzing and assembling large volumes of sequence data. 4) Fluorescent detection methods: The use of fluorescent dyes or other non-radioactive methods for detecting DNA sequences has replaced older radioactive methods, making the process safer and more efficient. These advances have collectively contributed to the development of high-throughput sequencing technologies.