Which is useful for high-throughput sequencing, but is not part of Sanger DNA sequencing?

Next-generation sequencing (NGS) technologies are specifically designed for high-throughput sequencing and are not part of traditional Sanger sequencing. Sanger sequencing relies on chain termination methods, where DNA fragments are synthesized one at a time and then read sequentially. In contrast, NGS methods use technologies such as sequencing by synthesis, sequencing by ligation, or ion semiconductor sequencing to process millions of DNA fragments simultaneously. This parallel processing capability is what enables the high throughput of NGS.