Where does glycolysis occur in eukaryotic cells?

Glycolysis occurs in the cytoplasm of eukaryotic cells. This includes both plant and animal cells. The enzymes that catalyze the reactions in glycolysis are located in the cytosol, and the process is independent of the mitochondria. The resulting pyruvate can then enter the mitochondria for aerobic respiration if oxygen is available, or it can undergo fermentation in the cytoplasm if oxygen is scarce.