Bacteriology 15 Views 1 Answers

Sourav Pan🥇 GoldDecember 7, 2024
What is the purpose of CaCl₂ in bacterial transformation?
What is the purpose of CaCl₂ in bacterial transformation?
Please login to save the post
1
Please login to submit an answer.

Sourav Pan🥇 GoldMay 15, 2025
Calcium chloride is used to make bacterial cells competent for DNA uptake by destabilizing the cell membrane. The calcium ions neutralize the negative charges on both the bacterial cell membrane and the DNA, reducing repulsion and facilitating binding. The treatment also makes the membrane more permeable, enabling DNA to enter the cells during the heat shock step.
0
0 likes
- Share on Facebook
- Share on Twitter
- Share on LinkedIn