Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷
Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)
(A) 10⁴
(B) 10⁵
(C) 10⁶
(D) 10⁷
Cite this post:
Sourav Pan. (2024, August 20). Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/receptor-r-is-over-expressed-in-cho-cells-and-analysed-for-expression-6-x-10%e2%81%b7-cells-were-incubated-with-its-radioactive-ligand-specific-activity-100-counts-per-picomole-if-the-total-c/
Sourav Pan. "Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷." Biology Notes Online, 20 August 2024, biologynotesonline.com/qa/receptor-r-is-over-expressed-in-cho-cells-and-analysed-for-expression-6-x-10%e2%81%b7-cells-were-incubated-with-its-radioactive-ligand-specific-activity-100-counts-per-picomole-if-the-total-c/.
Sourav Pan. "Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷." Biology Notes Online (blog). August 20, 2024. https://biologynotesonline.com/qa/receptor-r-is-over-expressed-in-cho-cells-and-analysed-for-expression-6-x-10%e2%81%b7-cells-were-incubated-with-its-radioactive-ligand-specific-activity-100-counts-per-picomole-if-the-total-c/.
Answer: (A) 10⁴
Total counts = 1000 cpm, and specific activity = 100 counts per picomole. Thus, total ligand bound = 1000 cpm / 100 counts per picomole = 10 picomoles. Given 6 × 10⁷ cells, average number of receptors per cell = 10 picomoles / 6 × 10⁷ cells = 10⁴ receptors per cell.
Cite this post:
Sourav Pan. (2024, August 20). Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/receptor-r-is-over-expressed-in-cho-cells-and-analysed-for-expression-6-x-10%e2%81%b7-cells-were-incubated-with-its-radioactive-ligand-specific-activity-100-counts-per-picomole-if-the-total-c/
Sourav Pan. "Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷." Biology Notes Online, 20 August 2024, biologynotesonline.com/qa/receptor-r-is-over-expressed-in-cho-cells-and-analysed-for-expression-6-x-10%e2%81%b7-cells-were-incubated-with-its-radioactive-ligand-specific-activity-100-counts-per-picomole-if-the-total-c/.
Sourav Pan. "Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor) (A) 10⁴ (B) 10⁵ (C) 10⁶ (D) 10⁷." Biology Notes Online (blog). August 20, 2024. https://biologynotesonline.com/qa/receptor-r-is-over-expressed-in-cho-cells-and-analysed-for-expression-6-x-10%e2%81%b7-cells-were-incubated-with-its-radioactive-ligand-specific-activity-100-counts-per-picomole-if-the-total-c/.
