How does cyanide affect glycolysis?

Cyanide affects glycolysis indirectly by inhibiting the mitochondrial electron transport chain, particularly by binding to cytochrome c oxidase (Complex IV). This blockage prevents the use of oxygen for aerobic respiration, but glycolysis itself can still proceed in the cytoplasm. However, in the long term, the inability to regenerate NAD+ through oxidative phosphorylation will hinder glycolysis.