Biochemistry 5 Views 1 Answers
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Sourav PanFebruary 8, 2025

How does a gaspak anaerobic system work?

How does a gaspak anaerobic system work?

Sourav Pan
Sourav PanFebruary 8, 2025

Answered

The GasPak anaerobic system works by removing oxygen from a sealed environment to create conditions suitable for anaerobic bacteria. Here’s a simple explanation of the process:

  • Preparation: Culture media or samples are placed inside an airtight jar along with an anaerobic indicator strip that changes color if oxygen is present.
  • Activation: A gas generator sachet is then added to the jar. When activated—often by adding water—the sachet’s chemicals (like sodium borohydride and sodium bicarbonate) react to produce hydrogen and carbon dioxide gases.
  • Oxygen Removal: The hydrogen gas reacts with any oxygen present in the jar. This reaction is typically catalyzed by a palladium catalyst, converting oxygen into water and thereby eliminating oxygen from the environment.
  • Confirmation: The anaerobic indicator strip is monitored. If the strip changes to a color that indicates low or no oxygen, it confirms that the jar is now anaerobic, creating the right conditions for the growth of anaerobic microorganisms.

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