Fluorescence Microscopy – Definition, Principle, Parts, Uses, Examples

Fluorescence Microscopy - Definition, Principle, Parts, Uses, Examples

The concept of using fluorescence to study biological materials dates back to the late 19th century, but the first practical fluorescence microscope was not developed until the 1930s. In 1938, Robert Goldstein and Richard Manly, researchers at the University of California, Berkeley, published a paper describing the use of fluorescence to study cells and tissues … Read more

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy - Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy, short for Ultraviolet-Visible Spectroscopy, is an analytical technique widely used in the fields of chemistry, physics, biochemistry, and molecular biology. It’s based on the absorption or transmission of UV and visible light by substances. At its core, UV-Vis Spectroscopy involves shining ultraviolet or visible light through a sample and measuring how much of … Read more

Objective Lenses – Principle, Types, Specification, Uses

Objective Lenses - Principle, Types, Specification, Uses

What is Objective Lens? Purpose of Objective Lenses Objective lenses serve several key purposes in optical instruments, playing a critical role in magnification, image formation, and resolution. The primary purposes of objective lenses include: Specification of Objective Lens The specification of an objective lens encompasses several key parameters that define its performance and suitability for … Read more

Microscope Condenser – Types, Parts, Mechanism, Uses

Microscope Condenser - Types, Parts, Mechanism, Uses

A microscope condenser is an integral optical component in the realm of microscopy, designed to focus and direct light onto the specimen being examined. This specialized lens system is strategically positioned beneath the microscope stage, a placement crucial for optimal illumination. The primary function of the condenser is to gather light from the microscope’s light … Read more

Parts of a Microscope and Their Functions

Parts of a Microscope and Their Functions

What are Microscopes? Parts of a Microscope The microscope parts are divided into two main categories,  such as; Structural parts of a microscope and their functions Structural parts of a microscope Location Function Head (Body Tube) Situated at the microscope’s apex. Serves as a pivotal connector, linking the eyepiece to the objective lenses, ensuring a … Read more

Ion Exchange Chromatography – Principle, Protocol, Applications, Examples

Ion Exchange Chromatography – Definition, Principle, Protocol, Applications, Examples

What is Ion Exchange Chromatography? Definition of Ion Exchange Chromatography Ion exchange chromatography is a separation technique that separates ions and polar molecules based on their affinity to ion exchangers. It involves the reversible exchange of ions between the target ions in the sample solution and ions attached to an ion exchanger. Working Principle of … Read more

Ion exchange mechanism in Ion Exchange Chromatography

Ion exchange mechanism in Ion Exchange Chromatography

Ion exchange chromatography (IEC) is a chromatographic technique that allows for the separation of ions and polar molecules based on their charge. It utilizes a stationary phase composed of an insoluble matrix with covalently bonded charged groups. These charged groups can attract and bind ions of the opposite charge present in the mobile phase, which … Read more

Ocular Micrometer – Definition, Principle, Parts, Applications

Ocular Micrometer - Definition, Principle, Parts, Applications

What is Ocular Micrometer? Working Principle of Ocular Micrometer The principle of an ocular micrometer revolves around using a calibrated scale to accurately measure the size of objects viewed through a microscope. This scale, typically divided into 100 divisions, each measuring 0.01 millimeters (µm), is an essential component placed within the eyepiece of the microscope. … Read more

Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×